Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Kidney Epithelial Cell, Cell Culture
DISEASE(S): Disease Free
SUBMITTER: Srgjan Chivchiristov
LAB HEAD: Michelle Louise Halls
PROVIDER: PXD011033 | Pride | 2018-10-11
REPOSITORIES: Pride
Civciristov Srgjan S Ellisdon Andrew M AM Suderman Ryan R Pon Cindy K CK Evans Bronwyn A BA Kleifeld Oded O Charlton Steven J SJ Hlavacek William S WS Canals Meritxell M Halls Michelle L ML
Science signaling 20181009 551
G protein-coupled receptors (GPCRs) are the largest class of cell surface signaling proteins, participate in nearly all physiological processes, and are the targets of 30% of marketed drugs. Typically, nanomolar to micromolar concentrations of ligand are used to activate GPCRs in experimental systems. We detected GPCR responses to a wide range of ligand concentrations, from attomolar to millimolar, by measuring GPCR-stimulated production of cyclic adenosine monophosphate (cAMP) with high spatial ...[more]