Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive
ORGANISM(S): Escherichia Coli Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Vadim Shchepachev
LAB HEAD: David Tollervey
PROVIDER: PXD011071 | Pride | 2019-03-26
REPOSITORIES: Pride
Molecular systems biology 20190408 4
The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)-associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA-associated protein purification (TRAPP) based on 254 nm UV crosslinking and purification of all RNA-protein complexes using silica beads. In a variant approach (PAR-TRAPP), RNAs were labelled with 4-thiouracil prior to 350 nm crosslinking. PAR-TRAPP in yeast identified hundr ...[more]