Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PPR proteins in Arabidopsis mitoribosomes

ABSTRACT: Investigation of the protein composition of mitoribosomal subunits in Arabidopsis thaliana by complexome profiling

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Culture, Leaf

SUBMITTER: Holger Eubel

LAB HEAD: Prof. Dr. Hans-Peter Braun

PROVIDER: PXD011088 | Pride | 2019-04-30

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Complexome Profiling Reveals Association of PPR Proteins with Ribosomes in the Mitochondria of Plants.

Rugen Nils N Straube Henryk H Franken Linda E LE Braun Hans-Peter HP Eubel Holger H

Molecular & cellular proteomics : MCP 20190425 7

Mitochondrial transcripts are subject to a wealth of processing mechanisms including cis- and trans-splicing events, as well as base modifications (RNA editing). Hundreds of proteins are required for these processes in plant mitochondria, many of which belong to the pentatricopeptide repeat (PPR) protein superfamily. The structure, localization, and function of these proteins is only poorly understood. Here we present evidence that several PPR proteins are bound to mitoribosomes in plants. A nov ...[more]

PMID: 31023727

Similar Datasets

Protein Interaction Patterns in Arabidopsis thaliana Leaf Mitochondria Change in Response to Illumination

Project description:Light is the most important cue for plant metabolism since its presence enables photosynthesis. Mitochondria respond to light conditions by adjusting flow through the citric acid cycle and the respiratory chain to support photosynthesis in the light and provide the cell with ATP in the light and in the dark. The data presented here serve in identifying changes in protein:protein interactions (PPIs) of Arabidopsis thaliana leaf mitochondria in response to illumination and pave the way towards an understanding how PPIs affect and regulate mitochondrial metabolism.

2021-05-17 | PXD019253 | Pride

Complexome Profiling of the thylakoid proteome from Arabidopsis thaliana

Project description:We here present an investigation of the Arabidopsis thaliana energy metabolism taking place in the chloroplasts. Thylakoids were purified from Arabidopsis thaliana leaves and membrane bound protein complexes characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome profiling approach. Proteins were systematically identified by label-free quantitative shotgun proteomics.

2022-08-18 | PXD035871 | Pride

Complexome Profiling of the mitochondrial proteome from Viscum album

Project description:Viscum album is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). We here present an investigation of the V. album energy metabolism taking place in mitochondria. Mitochondria were purified from young V. album leaves and membrane bound protein complexes characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome profiling approach. Proteins were systematically identified by label-free quantitative shotgun proteomics.

2023-10-24 | PXD041061 | Pride

Complexome Profiling of the mitochondrial proteome from Viscum album

Project description:Recent genomic studies revealed that mitochondria from mistletoe species appear to lack a major amount of genes, indicating a possible loss of the NADH ubiquinone oxidoreductase (complex I) which forms an essential part of the mitochondrial Oxidative Phosphorylation System (OXPHOS). This dataset is used for profiling of the mitochondrial complexome from European mistletoe, Viscum album and gives biochemical evidence for a lack of complex I as well as a unique composition of OXPHOS in Viscum album.

2018-04-23 | PXD008974 | Pride

Complexome Profiling of the thylakoid proteome from Viscum album

Project description:Viscum album is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). We here present an investigation of the V. album energy metabolism taking place in the chloroplasts. Thylakoids were purified from young V. album leaves and membrane bound protein complexes characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome profiling approach. Proteins were systematically identified by label-free quantitative shotgun proteomics.

2022-08-18 | PXD035825 | Pride

ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization

Project description:The phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development.

2022-08-25 | PXD031063 | Pride

Regulation of root greening by light and auxin and cytokinin signalling in Arabidopsis

Project description:For microarray analysis plants (Arabidopsis thaliana) were grown for 7 days in the presence or absence of 1 uM 6-benzyladenine following growth on MS medium for 7 days.

2011-12-31 | E-MEXP-3132 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and TROL (AT4G01050) knock-out plants

Project description:Here we describe TROL (thylakoid rhodanese-like protein), a nuclear-encoded component of thylakoid membranes that is required for sustaining efficient linear electron flow in vascular plants. Thus, TROL might represent a missing thylakoid membrane linker for the assembly of a ternary complex between FNR, ferredoxin and NADP+. Such a complex is necessary for maintaining photosynthetic redox poise and balancing between several alternative electron-transport pathways of oxygenic photosynthesis. TROL inactivation modulates the expression of 638 nuclear genes, revealing a novel retrograde signaling pathway. Chloroplasts are the major source of NADPH which is exported to the cytosol where it participates in various redox-dependent processes. TROL is most likely the first element in a metabolic signaling pathway which influences the expression of a large set of stress related genes. Among them are O-methyltransferases and anthocyanin 5-aromatic acyltransferase which are highly upregulated. Microarray analysis was performed in order to identify metabolic networks and pathways regulated by the deficient protein TROL. Comparing ATH1 Affymetrix data results, 317 genes were down-regulated and 321 genes were classified as up-regulated, amongst which mostly affected enzymes were catalyses, oxigenases, monooxygenases and reductases involved in the process of photosynthesis.

2009-08-20 | E-MEXP-1783 | biostudies-arrayexpress

Structural analysis of the intrinsically disordered splicing factor Spp2 and its complex with the DEAH-box ATPase Prp2

Project description:Structural analysis of spp2 bound to prp2 with chemical crosslinking and mass spectrometry

2020-05-27 | PXD015793 | Pride

PsbS interacting proteins - PsbS interactions involved in the activation of energy dissipation in Arabidopsis

Project description:PsbS Protein Interactions during Non-photochemical quenching The non-photochemical quenching of excitation energy (NPQ) describes a photoprotective mechanism in the antenna of PSII which dissipates excess excitation energy as heat at the level of 1Chl* and by that prevents the formation of singlet oxygen in PSII. Four different components contribute to NPQ, namely qT, qE, qZ and qI. Under saturating light conditions, the qE represents the dominant NPQ component; qE is based on a complex mechanism which strictly depends on the ΔpH across the thylakoid membrane, the PsbS protein and the xanthophyll zeaxanthin (Zx). The central role of PsbS in these processes is related to the function of PsbS as sensor of the lumen pH. However, the molecular basis of this central function and particularly the underlying interactions of PsbS with PSII antenna proteins that lead to energy quenching are largely unclear. In this work, we present an in vitro approach to identify protein transient interactions involving the PsbS protein during NPQ induction and relaxation in Arabidopsis thaliana, revealing its direct role in the formation of quenching sites in the antenna complexes of photosystem II.

2016-01-12 | PXD002160 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data