Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Petra Beli
LAB HEAD: Petra Beli
PROVIDER: PXD011108 | Pride | 2019-11-12
REPOSITORIES: Pride
Balmus Gabriel G Pilger Domenic D Coates Julia J Demir Mukerrem M Sczaniecka-Clift Matylda M Barros Ana C AC Woods Michael M Fu Beiyuan B Yang Fengtang F Chen Elisabeth E Ostermaier Matthias M Stankovic Tatjana T Ponstingl Hannes H Herzog Mareike M Yusa Kosuke K Martinez Francisco Munoz FM Durant Stephen T ST Galanty Yaron Y Beli Petra P Adams David J DJ Bradley Allan A Metzakopian Emmanouil E Forment Josep V JV Jackson Stephen P SP
Nature communications 20190108 1
Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging chemotherapeutic agents. To explore genetic resistance mechanisms, we performed genome-wide CRISPR-Cas9 screens in cells treated with the DNA topoisomerase I inhibitor topotecan. Thus, we here establish that inactivating terminal components of the non-homologous end-joining (NHEJ) machinery or of the BRCA1-A complex specifically confer topotecan resistance to ATM-deficient cells. We show that hypersensitivity of A ...[more]