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N-terminome Method Development (Discovery)

ABSTRACT: Developed a method to enrich N-terminome. Several parameters were change to optimization. This project focused on the discovery of N-terminome.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Shinyeong Ju

LAB HEAD: Cheolju Lee

PROVIDER: PXD011141 | Pride | 2020-05-25

REPOSITORIES: Pride

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iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform.

Ju Shinyeong S Kwon Yumi Y Kim Jeong-Mok JM Park Daechan D Lee Seonjeong S Lee Jin-Won JW Hwang Cheol-Sang CS Lee Cheolju C

Analytical chemistry 20200421 9

The field of terminal proteomics is limited in that it is optimized for large-scale analysis via multistep processes involving liquid chromatography. Here, we present an integrated N-terminal peptide enrichment method (iNrich) that can handle as little as 25 μg of cell lysate via a single-stage encapsulated solid-phase extraction column. iNrich enables simple, rapid, and reproducible sample processing, treatment of a wide range of protein amounts (25 μg ∼ 1 mg), multiplexed parallel sample prepa ...[more]

PMID: 32267142

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| MSV000080204 | MassIVE

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