Proteomics

Dataset Information

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Robust, reproducible, and economical phosphopeptide enrichment using calcium titanate.

ABSTRACT: We compare different preparations of TiO2 for phosphopeptide enrichment and demonstrate the efficacy of a method using CaTiO3

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Acute Leukemia,Cervix Carcinoma

SUBMITTER: Noah Dephoure  

LAB HEAD: Noah Dephoure

PROVIDER: PXD011145 | Pride | 2019-01-02

REPOSITORIES: Pride

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Publications

Robust, Reproducible, and Economical Phosphopeptide Enrichment Using Calcium Titanate.

Ahmed Adnan A   Raja Vijay J VJ   Cavaliere Paola P   Dephoure Noah N  

Journal of proteome research 20190103 3

Mass-spectrometry-based phosphoproteomics has revolutionized phosphoprotein analysis and enhanced our understanding of diverse and fundamental cellular processes important for human health and disease. Because of their relative scarcity, phosphopeptides must be enriched before analysis. Many different enrichment methods and materials have been described, and many reports have made claims about the advantages of particular materials and methodological variations. We demonstrate an effective and h  ...[more]