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Multiplex PRM quantitation of CD8a, CD4, LAG3, PD1, PD-L1 and PD-L2

ABSTRACT: Immuno-LC-PRM assay was developed to simultaneously quantify the expression levels of six immune markers (CD8A, CD4, LAG3, PD1, PD-L1 and PD-L2) using as little as 1-2 mg of fresh frozen tissue.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Lymph Node

DISEASE(S): Cervix Carcinoma

SUBMITTER: Yashu Liu

LAB HEAD: Yashu Liu

PROVIDER: PXD011152 | Pride | 2018-10-04

REPOSITORIES: Pride

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Multiplex Immuno-Liquid Chromatography-Mass Spectrometry-Parallel Reaction Monitoring (LC-MS-PRM) Quantitation of CD8A, CD4, LAG3, PD1, PD-L1, and PD-L2 in Frozen Human Tissues.

Zhang Qian Q Salzler Robert R Dore Anthony A Yang Janice J Ma Dangshe D Olson William C WC Liu Yashu Y

Journal of proteome research 20181011 11

The immune status of tumors critically influences their responsiveness to PD1 blockades and other immune-based therapies. Programmed death ligand 1 (PD-L1) immunohistochemistry (IHC) is a clinically validated predictive biomarker of response to checkpoint-inhibitor therapy in a limited number of clinical settings but is poorly predictive in most. With emerging evidence that multiple pathways and immune-checkpoint proteins may coordinately contribute to the adaptive immune resistance, the identif ...[more]

PMID: 30277784

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