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Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity

ABSTRACT: We comprehensively profiled intracellular and ECM proteomes of S. aureus flow biofilms and complemented these data by metabolic footprint analysis and phenotypic assays. We show that S. aureus biofilms produce high amounts of secreted, moonlighting virulence factors stabilizing the ECM. Mechanistically, we propose that these alkaline virulence factors get protonated in an acidified ECM (due to the release of acids upon fermentation) mediating electrostatic interactions with anionic cell surface components and metabolites, which leads to cell aggregation and ECM stabilization.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus Subsp. Aureus Nctc 8325

SUBMITTER: Daniela Zuehlke

LAB HEAD: Riedel, Katharina

PROVIDER: PXD011157 | Pride | 2019-03-13

REPOSITORIES: Pride

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Virulence Factors Produced by Staphylococcus aureus Biofilms Have a Moonlighting Function Contributing to Biofilm Integrity.

Graf Alexander C AC Leonard Anne A Schäuble Manuel M Rieckmann Lisa M LM Hoyer Juliane J Maass Sandra S Lalk Michael M Becher Dörte D Pané-Farré Jan J Riedel Katharina K

Molecular & cellular proteomics : MCP 20190308 6

Staphylococcus aureus is the causative agent of various biofilm-associated infections in humans causing major healthcare problems worldwide. This type of infection is inherently difficult to treat because of a reduced metabolic activity of biofilm-embedded cells and the protective nature of a surrounding extracellular matrix (ECM). However, little is known about S. aureus biofilm physiology and the proteinaceous composition of the ECM. Thus, we cultivated S. aureus biofilms ...[more]

PMID: 30850421

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