Dataset Information

Proteome and phosphoproteome dynamics of CVB3 infected cells

ABSTRACT: Viruses are obligate intracellular parasites that reshape the ultrastructure, composition and metabolism of host cells to suit their specific needs, although disparate viruses may modify their host in different ways. Such changes may be specifically induced by the virus to support the infection or be part of cellular antiviral responses or stress responses. Here, using state-of-the-art quantitative (phospho)proteomics, we reveal the unique proteome and phosphoproteome dynamics that occur in the host cells infected with the enterovirus coxsackievirus B3 (CVB3).

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human) Coxsackievirus B3

TISSUE(S): Cell Culture

SUBMITTER: Piero Giansanti

LAB HEAD: Albert J. R. Heck

PROVIDER: PXD011163 | Pride | 2020-07-23

REPOSITORIES: Pride

ACCESS DATA

Publications

Dynamic remodelling of the human host cell proteome and phosphoproteome upon enterovirus infection.

Giansanti Piero P Strating Jeroen R P M JRPM Defourny Kyra A Y KAY Cesonyte Ieva I Bottino Alexia M S AMS Post Harm H Viktorova Ekaterina G EG Ho Vien Quang Tri VQT Langereis Martijn A MA Belov George A GA Nolte-'t Hoen Esther N M ENM Heck Albert J R AJR van Kuppeveld Frank J M FJM

Nature communications 20200828 1

The group of enteroviruses contains many important pathogens for humans, including poliovirus, coxsackievirus, rhinovirus, as well as newly emerging global health threats such as EV-A71 and EV-D68. Here, we describe an unbiased, system-wide and time-resolved analysis of the proteome and phosphoproteome of human cells infected with coxsackievirus B3. Of the ~3,200 proteins quantified throughout the time course, a large amount (~25%) shows a significant change, with the majority being downregulate ...[more]

PMID: 32859902

Similar Datasets

Project description:Cyanobacteria are highly abundant in the oceans where they are constantly exposed to lytic viruses. Some viruses are restricted to a narrow host range while others infect a broad range of hosts. It is currently unknown whether broad-host range phages employ the same infection program, or regulate their program in a host-specific manner to accommodate for the different genetic makeup and defense systems of each host. Here we used a combination of microarray and RNA-seq analyses to investigate the interaction of three phylogentically distinct Synechococcus strains, WH7803, WH8102, and WH8109, with the broad-host range T4-like myovirus, Syn9, during infection. Strikingly, we found that the phage led a nearly identical expression program in the three hosts despite considerable differences in host gene content. On the other hand, host responses to infection involved mainly host-specific genes, suggesting variable attempts at defense against infection. A large number of responsive host genes were located in hypervariable genomic islands, substantiating genomic islands as a major axis of phage-bacteria interactions in cyanobacteria. Furthermore, transcriptome analyses and experimental determination of the complete phage promoter map revealed three temporally regulated modules and not two as previously thought for cyanophages. In contrast to T4, an extensive, previously unknown regulatory motif drives expression of early genes and host-like promoters drive middle-gene expression. These promoters are highly conserved among cyanophages and host-like middle promoters extend to other T4-like phages, indicating that the well-known mode of regulation in T4 is not the rule among the broad family of T4-like phages. We investigated the infection process and transcriptional program of the P-TIM40 cyanophage during infection of a Prochlorococcus NATL2A host. The results are discussed in conjunction with results obtained from the infection process for the Syn9 cyanophage in three different Synechococcus hosts: WH7803 (Dufresne et al. 2008), WH8102 (Palenik et al. 2003) and WH8109 (sequenced as part of this study).

Dataset Information

Proteome and phosphoproteome dynamics of CVB3 infected cells

Publications

Dynamic remodelling of the human host cell proteome and phosphoproteome upon enterovirus infection.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure