Ontology highlight
ABSTRACT:
INSTRUMENT(S): Bruker Daltonics micrOTOF series
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell, Hela Cell
SUBMITTER: Laura Dagley
LAB HEAD: Andrew I Webb
PROVIDER: PXD011169 | Pride | 2019-06-05
REPOSITORIES: Pride
Dagley Laura F LF Infusini Giuseppe G Larsen Rune H RH Sandow Jarrod J JJ Webb Andrew I AI
Journal of proteome research 20190610 7
Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical to the success of quantitative workflows. Here we present a universal, solid-phase protein preparation (USP<sup>3</sup>) method which is rapid, robust, and scalable, facilitating high-throughput protein sample preparation for bottom-up and top-down mass spectrometry (MS) analysis. This technique builds upon the single-pot solid-phase-enhanced sample preparation (SP3) where we now demonstrate its scalabilit ...[more]