Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Central bearded dragon hibernation label-free LC-MS/MS

ABSTRACT: Hibernation is a complex physiological state often exploited by animals under adverse environmental conditions. It involves large changes in metabolism and cellular function, with many stress responses modulated to tolerate physiological challenges that would otherwise be lethal. This study explores transcriptomic and proteomic profiling of hibernation in a reptile (the central bearded dragon; Pogona vitticeps) throughout the hibernation season. To better understand molecular dynamics of this remarkable process, total RNA and protein was analyzed in brain, heart and skeletal muscle at three time points; late hibernation, two days post-arousal, and two months post-arousal.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Pogona Vitticeps

TISSUE(S): Heart, Brain, Skeletal Muscle

SUBMITTER: Alexander Capraro

LAB HEAD: Paul D Waters

PROVIDER: PXD011171 | Pride | 2019-04-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Analysis of molecular signatures in mammalian hibernation

Project description:Mammalian hibernators display phenotypes similar to physiological conditions in non-hibernating species under conditions of calorie restriction and fasting, hypoxia, hypothermia, ischemia-reperfusion, and sleep. However, whether or how similarities are also reflected on molecular and genetic levels is unclear. We identified molecular signatures of torpor and arousal in hibernation using a new custom-designed cDNA microarray for the arctic ground squirrel (Urocitellus parryii,) and compared them to molecular signatures of selected phenotypes in mouse. Our results show that differential gene expression related to metabolism during torpor is closely related to that during calorie restriction and hypoxia. PPARM-NM-1 is crucial for metabolic remodeling in hibernation. Genes related to the sleep-wake cycle and temperature response genes induced by hypothermia follow the same expression changes as in torpor-arousal cycle. Increased fatty acid metabolism might contribute to the protection against ischemia-reperfusion injury during hibernation. Further, by comparing with thousands of pharmacological signatures, we identified drugs that may induce similar expression patterns in human cell lines as during hibernation. Arctic ground squirrels sampled during winter hibernation were compared with the animals sampled during summer. Liver was hybridized on a custom 9,600 probes nylon membrane microarray platform. Four squirrels in early torpor, five in late torpor, four in early arousal, four in late arousal, and seven in summer active were studied in experiments.

2012-09-01 | E-GEOD-38700 | biostudies-arrayexpress

Comparative transcriptomics of the garden dormouse hypothalamus during early torpor, late torpor and interbout arousal of hibernation

Project description:Comparative transcriptomics of the garden dormouse hypothalamus during early torpor, late torpor and interbout arousal of hibernation

2023-11-13 | GSE207494 | GEO

Golden-mantled ground squirrel (Spermophilus lateralis) hibernating heart transcriptome

Project description:This series represents the heart transcriptome for animals sampled during summer, interbout arousal, and late torpor Keywords = squirrel, heart, hibernation Keywords: other

2004-12-03 | GSE2023 | GEO

13-lined ground squirrel (Spermophilus tridecemlineatus) hibernating liver transcriptome

Project description:This series represents the liver transcriptome for animals sampled during summer, interbout arousal, and late torpor Keywords = squirrel, liver, hibernation Keywords: other

2004-12-03 | GSE2021 | GEO

13-lined ground squirrel (Spermophilus tridecemlineatus) hibernating liver transcriptome

Project description:This series represents the liver transcriptome for animals sampled during summer, interbout arousal, and late torpor Keywords = squirrel, liver, hibernation

2010-06-05 | E-GEOD-2021 | biostudies-arrayexpress

Golden-mantled ground squirrel (Spermophilus lateralis) hibernating heart transcriptome

Project description:This series represents the heart transcriptome for animals sampled during summer, interbout arousal, and late torpor Keywords = squirrel, heart, hibernation

2010-06-05 | E-GEOD-2023 | biostudies-arrayexpress

Golden-mantled ground squirrel (Spermophilus lateralis) hibernating liver transcriptome

Project description:This series represents the liver transcriptome for animals sampled during summer, interbout arousal, and late torpor Keywords = squirrel Keywords = liver Keywords = hibernation

2010-06-05 | E-GEOD-2024 | biostudies-arrayexpress

Golden-mantled ground squirrel (Spermophilus lateralis) hibernating liver transcriptome

2004-12-03 | GSE2024 | GEO

Analysis of molecular signatures in mammalian hibernation

Project description:Mammalian hibernators display phenotypes similar to physiological conditions in non-hibernating species under conditions of calorie restriction and fasting, hypoxia, hypothermia, ischemia-reperfusion, and sleep. However, whether or how similarities are also reflected on molecular and genetic levels is unclear. We identified molecular signatures of torpor and arousal in hibernation using a new custom-designed cDNA microarray for the arctic ground squirrel (Urocitellus parryii,) and compared them to molecular signatures of selected phenotypes in mouse. Our results show that differential gene expression related to metabolism during torpor is closely related to that during calorie restriction and hypoxia. PPARα is crucial for metabolic remodeling in hibernation. Genes related to the sleep-wake cycle and temperature response genes induced by hypothermia follow the same expression changes as in torpor-arousal cycle. Increased fatty acid metabolism might contribute to the protection against ischemia-reperfusion injury during hibernation. Further, by comparing with thousands of pharmacological signatures, we identified drugs that may induce similar expression patterns in human cell lines as during hibernation.

2012-09-01 | GSE38700 | GEO

Golden-mantled ground squirrel (Spermophilus lateralis) hibernating brain transcriptome

Project description:This series represents the brain transcriptome for animals sampled during summer, interbout arousal, and late torpor. Note that the brain expression data for interbout aroused animals was not analyzed in our publication because the group consisted of just 2 animals. Keywords = squirrel, brain, hibernation

2010-06-05 | E-GEOD-2022 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data