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Proteomic profiling of RNase III-dependent asRNA regulation in E. coli

ABSTRACT: In an effort to determine if the RNase III-dependent asRNA regulation had an effect on protein production in E. coli we conducted two TMT 6-plex experiments comparing cultures of wildtype E. coli, rnc-14, rnc-38, hfq in the stationary and exponential phase.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Nancy Finkel

LAB HEAD: Nancy Finkel

PROVIDER: PXD011180 | Pride | 2020-06-18

REPOSITORIES: Pride

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Tombusvirus p19 Captures RNase III-Cleaved Double-Stranded RNAs Formed by Overlapping Sense and Antisense Transcripts in Escherichia coli.

Huang Linfeng L Deighan Padraig P Jin Jingmin J Li Yingxue Y Cheung Hung-Chi HC Lee Elaine E Mo Shirley S SS Hoover Heather H Abubucker Sahar S Finkel Nancy N McReynolds Larry L Hochschild Ann A Lieberman Judy J

mBio 20200609 3

Antisense transcription is widespread in bacteria. By base pairing with overlapping sense RNAs, antisense RNAs (asRNA) can form double-stranded RNAs (dsRNA), which are cleaved by RNase III, a dsRNA endoribonuclease. The ectopic expression of plant Tombusvirus p19 in Escherichia coli stabilizes ∼21-nucleotide (nt) dsRNA RNase III decay intermediates, which enabled us to characterize otherwise highly unstable asRNA by deep sequencing of p19-captured dsRNA. RNase III-produced small ds ...[more]

PMID: 32518184

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