Sample Preparation by Easy Extraction and Digestion (SPEED) - A Universal, Rapid, and Detergent-free Protocol for Proteomics based on Acid Extraction

ABSTRACT: We report a universal detergent-free method, named Sample Preparation by Easy Extraction and Digestion (SPEED), which consists of three mandatory steps, acidification, neutralization and digestion. SPEED is a universal method for peptide generation from various sources and is easily applicable even for lysis-resistant sample types. The protocol is highly reproducible, virtually loss-less and enables very rapid sample processing. SPEED is superior to detergent/chaotropic agent-based methods for quantitative proteomics and enhances proteome coverage for challenging samples.

INSTRUMENT(S):

ORGANISM(S): Bacillus Cereus Atcc 10987 Homo Sapiens (human) Escherichia Coli Staphylococcus Aureus Mus Musculus (mouse)

TISSUE(S): Lung

SUBMITTER: Joerg Doellinger  

LAB HEAD: Peter Lasch

PROVIDER: PXD011189 | Pride | 2019-11-25

REPOSITORIES: Pride