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Pichia pastoris organelles by LC-ESI-MS

ABSTRACT: Organelles from Pichia, either grown on glucose or methanol were purified and analysed using LC-ESI-MS. Two biological replicates each had been analyzed leading to four replicate data sets for every organelle. Organelles analyzed: cis golgi, trans golgi, plasma membrane, microsomes, cytosol, vacuole, peroxisomes and mitochondria

INSTRUMENT(S): maXis

ORGANISM(S): Komagataella Pastoris (yeast) (pichia Pastoris)

SUBMITTER: Clemens Grünwald-Gruber

LAB HEAD: Friedrich Altmann

PROVIDER: PXD011220 | Pride | 2020-05-26

REPOSITORIES: Pride

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A subcellular proteome atlas of the yeast Komagataella phaffii.

Valli Minoska M Grillitsch Karlheinz K Grünwald-Gruber Clemens C Tatto Nadine E NE Hrobath Bernhard B Klug Lisa L Ivashov Vasyl V Hauzmayer Sandra S Koller Martina M Tir Nora N Leisch Friedrich F Gasser Brigitte B Graf Alexandra B AB Altmann Friedrich F Daum Günther G Mattanovich Diethard D

FEMS yeast research 20200201 1

The compartmentalization of metabolic and regulatory pathways is a common pattern of living organisms. Eukaryotic cells are subdivided into several organelles enclosed by lipid membranes. Organelle proteomes define their functions. Yeasts, as simple eukaryotic single cell organisms, are valuable models for higher eukaryotes and frequently used for biotechnological applications. While the subcellular distribution of proteins is well studied in Saccharomyces cerevisiae, this is not the case for ot ...[more]

PMID: 31922548

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