Proteomics

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GPR35 promotes glycolysis, proliferation and oncogenic signalling by engaging with the sodium potassium pump

ABSTRACT: We used human embryonic kidney HEK293T cells transfected with human GPR35 cDNAs as baits that were engineered to express Strep or hemagglutinin (HA) tags at the C terminus to perform an unbiased proteomics survey for GPR35 interaction partners.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Georg Schneditz  

LAB HEAD: Arthur Kaser

PROVIDER: PXD011269 | Pride | 2019-11-12

REPOSITORIES: Pride

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The sodium potassium pump (Na/K-ATPase) ensures the electrochemical gradient of a cell through an energy-dependent process that consumes about one-third of regenerated ATP. We report that the G protein-coupled receptor GPR35 interacted with the α chain of Na/K-ATPase and promotes its ion transport and Src signaling activity in a ligand-independent manner. Deletion of Gpr35 increased baseline Ca<sup>2+</sup> to maximal levels and reduced Src activation and overall metabolic activity in macrophage  ...[more]

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