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The dynamic proteome of Influenza A virus infection

ABSTRACT: We combined metabolic pulse labeling and quantitative shotgun proteomics to globally monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain. While production of host proteins was remarkably similar, we observed striking differences in the kinetics of viral protein synthesis over the course of infection. Most importantly, the matrix protein M1 was inefficiently produced by the bird-adapted strain at later stages.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive Plus, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Influenza

SUBMITTER: Boris Bogdanow

LAB HEAD: Matthias Selbach

PROVIDER: PXD011321 | Pride | 2019-11-11

REPOSITORIES: Pride

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The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant.

Bogdanow Boris B Wang Xi X Eichelbaum Katrin K Sadewasser Anne A Husic Immanuel I Paki Katharina K Budt Matthias M Hergeselle Martha M Vetter Barbara B Hou Jingyi J Chen Wei W Wiebusch Lüder L Meyer Irmtraud M IM Wolff Thorsten T Selbach Matthias M

Nature communications 20191204 1

Pandemic influenza A virus (IAV) outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular basis of this species barrier is incompletely understood. Here we combine metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain and observe striking differences in viral protein synthesis. Most importantly, the matrix protein M1 is inefficientl ...[more]

PMID: 31797923

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