Proteomics,Multiomics

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KRAB Zincfinger Interactome - The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification

ABSTRACT: Krüppel-associated box (KRAB)-containing zinc finger proteins (KZFPs) represent the largest family of human transcription factors. The majority of its members bind to Transposable Elements (TEs), which they repress through the recruitment of the repressor KAP1 to their conserved KRAB domain. In addition to this recognized role, some KZFPs seem to favor different types of loci such as transcription start sites or display diverse functions such as imprinting and gene regulation. Intriguingly, a subset of KZFPs was shown not to recruit the corepressor KAP1. Therefore, we sought to get a better picture of KZFPs putative roles and their association with KAP1. In order to do so, we generated the interactomes of 101 KZFPs through Affinity Purification followed by Mass Spectrometry (AP-MS).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Moritz Heusel  

LAB HEAD: Rudolf Aebersold

PROVIDER: PXD011322 | Pride | 2019-06-04

REPOSITORIES: Pride

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The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification.

Helleboid Pierre-Yves PY   Heusel Moritz M   Duc Julien J   Piot Cécile C   Thorball Christian W CW   Coluccio Andrea A   Pontis Julien J   Imbeault Michaël M   Turelli Priscilla P   Aebersold Ruedi R   Trono Didier D  

The EMBO journal 20190812 18

Krüppel-associated box (KRAB)-containing zinc finger proteins (KZFPs) are encoded in the hundreds by the genomes of higher vertebrates, and many act with the heterochromatin-inducing KAP1 as repressors of transposable elements (TEs) during early embryogenesis. Yet, their widespread expression in adult tissues and enrichment at other genetic loci indicate additional roles. Here, we characterized the protein interactome of 101 of the ~350 human KZFPs. Consistent with their targeting of TEs, most K  ...[more]

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