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On-chip AP-MS: Interaction proteomics on a microfluidics platform

ABSTRACT: Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of input lysate, representing a ~50-100 fold downscaling compared to regular microcentrifuge tubes-based workflows. As such, our method holds great promise for future AP-MS applications in which sample amounts are limited.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Cristina Furlan

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD011323 | Pride | 2022-03-10

REPOSITORIES: Pride

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On-chip AP-MS: Interaction proteomics on a microfluidics platform

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