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Ultraviolet Photodissociation of ESI and MALDI generated protein ions on a Q-Exactive mass spectrometer

ABSTRACT: UVPD was implemented on an Orbitrap Q-Exactive plus equipped with a ESI/EP-MALDI. UVPD of MALDI generated ions was benchmarked against MALDI-ISD, MALDI-HCD and ESI-UVPD. MALDI UVPD outperformed MALDI HCD and ISD efficiently sequencing small proteins ions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse) Homo Sapiens (human)

SUBMITTER: Marialaura Dilillo

LAB HEAD: Liam A. McDonnell

PROVIDER: PXD011526 | Pride | 2019-01-24

REPOSITORIES: Pride

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Ultraviolet Photodissociation of ESI- and MALDI-Generated Protein Ions on a Q-Exactive Mass Spectrometer.

Dilillo Marialaura M de Graaf Erik L EL Yadav Avinash A Belov Mikhail E ME McDonnell Liam A LA

Journal of proteome research 20181204 1

The identification of molecular ions produced by MALDI or ESI strongly relies on their fragmentation to structurally informative fragments. The widely diffused fragmentation techniques for ESI multiply charged ions are either incompatible (ECD and ETD) or show lower efficiency (CID, HCD), with the predominantly singly charged peptide and protein ions formed by MALDI. In-source decay has been successfully adopted to sequence MALDI-generated ions, but it further increases spectral complexity, and ...[more]

PMID: 30484663

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