Proteomics

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SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity


ABSTRACT: Arabidopsis lines expressing 35S::Strep-SUMO1H89R in Col-0 background were generated since the H89R SUMO1 variant simplifies MS/MS detection of SUMO conjugated lysines after trypsinization (Miller et al., 2010). Transgenic seedlings were treated with water or 250nM flg22 for 30 minutes and total protein extracts including membrane fractions were affinity purified with Pierce™ Streptavidin Magnetic Beads. LC-MS analysis of eluting proteins allowed identification of candidate SUMO-conjugated proteins in response to flg22 treatment.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: Adrian Brown  

LAB HEAD: Ari Sadanandom

PROVIDER: PXD011552 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity.

Orosa Beatriz B   Yates Gary G   Verma Vivek V   Srivastava Anjil K AK   Srivastava Moumita M   Campanaro Alberto A   De Vega Daniel D   Fernandes Alanna A   Zhang Cunjin C   Lee Jack J   Bennett Malcolm J MJ   Sadanandom Ari A  

Nature communications 20181205 1


Detection of conserved microbial patterns by host cell surface pattern recognition receptors (PRRs) activates innate immunity. The FLAGELLIN-SENSITIVE 2 (FLS2) receptor perceives bacterial flagellin and recruits another PRR, BAK1 and the cytoplasmic-kinase BIK1 to form an active co-receptor complex that initiates antibacterial immunity in Arabidopsis. Molecular mechanisms that transmit flagellin perception from the plasma-membrane FLS2-associated receptor complex to intracellular events are less  ...[more]

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