A ¨omic¨ approach to Pyrocystis lunula

ABSTRACT: Pyrocystis lunula (Schutt) is a photoautotrophic unarmoured dinoflagellate, commonly found in marine environments. Today it exist several biotechnological applications derived from the bioluminescent system of this species. From a post-genomic perspective, and in order to study the whole proteome of P. lunula, a ¨omic¨ approach (transcriptomic-proteomic analysis) was initiated using fresh microalgae samples. A total of 80.875.390 reads were generated (400.000 conting, XXX pb) and 17.461 peptides were detected, getting 3.182 protein identification hits. The identified proteins were categorized according to functional description and gene ontology classification. In this study, it has been developed and described the first proteomic analysis of the microalgae P. lunula. In addition to shed light on a series of important factors involved with the processes of regulation of gene expression. The presence of the luciferin-binding protein (LBP), which had not been described so far in Pyrocystis, is highlighted.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Eukaryota Pyrocystis Lunula

TISSUE(S): Cell Culture

SUBMITTER: Francisco Fernández-Acero  

LAB HEAD: Francisco Javier Fernández Acero

PROVIDER: PXD011555 | Pride | 2019-04-12

REPOSITORIES: Pride