LC-MSMS of RIBOPROTEOMES OF HEALTHY, POTATO VIRUS A- AND AGROBACTERIUM -INFECTED NICOTIANA BENTHAMIANA PLANTS

ABSTRACT: Nicotiana benthamiana is an important model plant for plant-microbe interaction studies. We compared the proteomes of ribosomes purified from healthy N. benthamiana plants and plants that were infected with two plant pathogens potato virus A (PVA genus Potyvirus) and A. tumefaciens bacteria. Ribosomes were affinity purified from transgenic leaves that expressed FLAG-tagged RPL18B (RPL: ribosome protein large subunit) of A. thaliana. Control purifications were made from non-transgenic plants that were infected with PVA. Our riboproteome revealed ~6600 r-protein hits representing 424 distinct r-proteins that were members from 71 of the expected 81 r-protein families.

INSTRUMENT(S):

ORGANISM(S): Potato Virus A Rhizobium Radiobacter (agrobacterium Tumefaciens) (agrobacterium Radiobacter) Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Viral Infectious Disease

SUBMITTER: Katri Eskelin  

LAB HEAD: Katri Eskelin

PROVIDER: PXD011602 | Pride | 2018-12-05

REPOSITORIES: Pride