Proteomic Characterization of Bradyrhizobium diazoefficiens bacteroids during soybean root nodules senescence

ABSTRACT: The form and physiology of Bradyrhizobium diazoefficiens after the decline of symbiotic nitrogen fixation has been characterized. Proteomic analyses showed that the post-symbiotic B. diazoefficiens undergo metabolic remodeling as well defined groups of proteins declined, increased or remained unchanged from 56 to 119 days after planting suggesting a transition to a hemibiotrophic-like lifestyle. Enzymatic analysis showed distinct patterns in both the cytoplasm and the periplasm. Similar to the bacteroid, the post-symbiotic bacteria rely on a non-citric acid cycle supply of succinate and although viable, they did not demonstrate the ability to grow within the senescent nodule.

INSTRUMENT(S):

ORGANISM(S): Bradyrhizobium Japonicum Usda 110

TISSUE(S): Plant Cell, Root Nodule

SUBMITTER: David Emerich  

LAB HEAD: David W. Emerich

PROVIDER: PXD011604 | Pride | 2019-11-12

REPOSITORIES: Pride