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Identification of HIPs in the islets of mice and non-diabetic human donors

ABSTRACT: Hybrid insulin peptides (HIPs) result from the linkage of an insulin C-peptide fragment and another peptide via a traditional peptide bond to generate a sequence that is not encoded in the genome. Here, we sought to identify HIPs naturally present in the pancreatic islets of non-obese diabetic (NOD) mice, BALB/c mice, and non-diabetic human donors by mass spectrometry.

INSTRUMENT(S): Agilent instrument model

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Pancreatic Islet, Islet Of Langerhans

DISEASE(S): Disease Free

SUBMITTER: Timothy Wiles

LAB HEAD: Thomas Delong

PROVIDER: PXD011606 | Pride | 2019-01-07

REPOSITORIES: Pride

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Identification of Hybrid Insulin Peptides (HIPs) in Mouse and Human Islets by Mass Spectrometry.

Wiles T. Aaron TA Powell Roger R Michel Cole R Beard K. Scott KS Hohenstein Anita A Bradley Brenda B Reisdorph Nichole N Haskins Kathryn K Delong Thomas T

Journal of proteome research 20190103 3

We recently discovered hybrid insulin peptides (HIPs) as a novel class of post-translationally modified peptides in murine-derived beta cell tumors, and we demonstrated that these molecules are autoantigens in type 1 diabetes (T1D). A HIP consists of an insulin fragment linked to another secretory granule peptide via a peptide bond. We verified that autoreactive CD4 T cells in both mouse and human autoimmune diabetes recognize these modified peptides. Here, we use mass spectrometric analyses to ...[more]

PMID: 30585061

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