IP-MS target protein identification

ABSTRACT: To identify the target of the monoclonal antibody, Immuoprecipitation (IP) was performed to pull-down target protein from cell culture extraction, corn or zebrafish tissue lysate. The IP product was validated on silver-stained SDS-PAGE and Western blot. The target band was cut out and performed LC-MS/MS for target protein identification.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Danio Rerio (zebrafish) (brachydanio Rerio) Zea Mays (maize)

TISSUE(S): Heart, Seed, Cell Culture

SUBMITTER: Yang Li  

LAB HEAD: Sheng-Ce Tao

PROVIDER: PXD011629 | Pride | 2020-05-26

REPOSITORIES: Pride