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Large-scale Profiling of ADP-Ribosylome by Chemical Proteomics

ABSTRACT: We have developed a dual metabolic incorporation of clickable NAD precursor compounds in live mammalian cells and in combination with the tandem mass tag (TMT) isobaric mass spectrometry, we have quantified the responses of thousands of endogenous proteins to clinical PARP inhibitors Olaparib and Rucaparib.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Kalesh Karunakaran

LAB HEAD: Peter A. DiMaggio

PROVIDER: PXD011631 | Pride | 2019-05-01

REPOSITORIES: Pride

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