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Nitric oxide-dependent anaerobic ammonium oxidation


ABSTRACT: Nitric oxide (NO) has several important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Even though NO is a potent oxidant, and was available on earth earlier than oxygen, its direct use by microorganisms for growth was not demonstrated before. Using physiological experiments, metatranscriptomics and metaproteomics, here we show that anaerobic ammonium-oxidizing (anammox) bacterium Kuenenia stuttgartiensis grow by coupling ammonium oxidation to NO reduction, and produce only N2. Such a metabolism could have existed on early earth, and has implications in controlling N2O and NO emissions both from natural and manmade ecosystems, where anammox bacteria contribute significantly to N2 release to the atmosphere.

INSTRUMENT(S): maXis

ORGANISM(S): Candidatus Kuenenia Stuttgartiensis

SUBMITTER: Hans Wessels  

LAB HEAD: Alain J van Gool

PROVIDER: PXD011763 | Pride | 2019-02-26

REPOSITORIES: Pride

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Publications

Nitric oxide-dependent anaerobic ammonium oxidation.

Hu Ziye Z   Wessels Hans J C T HJCT   van Alen Theo T   Jetten Mike S M MSM   Kartal Boran B  

Nature communications 20190320 1


Nitric oxide (NO) has important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N<sub>2</sub>O). Although NO is a potent oxidant, and was available on Earth earlier than oxygen, it is unclear whether NO can be used by microorganisms for growth. Anaerobic ammonium-oxidizing (anammox) bacteria couple nitrite reduction to ammonium oxidation with NO and hydrazine as intermediates, and produce  ...[more]

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