Proteomics

Dataset Information

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Fibroblast secretome proteomic analysis


ABSTRACT: LV fibroblasts were isolated from control (day 0), and infarcted regions at day 1, day 3, and day 7 after myocardial infarction (MI). Cells were isolated in DMEM in 10% fetal bovine serum and were seeded into 6-well plates and were allowed to grow to 80% confluence in DMEM supplemented with 10% fetal bovine serum (FBS). Cells were serum starved (DMEM + 0.1% FBS) for 18 h and then media was changed and secretome was collected after 24 h. The sample sizes are n=3 biological replicates for each time point.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart, Fibroblast

SUBMITTER: Elizabeth Flynn  

LAB HEAD: Merry Lindsey

PROVIDER: PXD011778 | Pride | 2019-10-25

REPOSITORIES: Pride

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Publications

Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis.

Mouton Alan J AJ   Ma Yonggang Y   Rivera Gonzalez Osvaldo J OJ   Daseke Michael J MJ   Flynn Elizabeth R ER   Freeman Tom C TC   Garrett Michael R MR   DeLeon-Pennell Kristine Y KY   Lindsey Merry L ML  

Basic research in cardiology 20190111 2


Cardiac fibroblasts are the major producers of extracellular matrix (ECM) to form infarct scar. We hypothesized that fibroblasts undergo a spectrum of phenotype states over the course of myocardial infarction (MI) from early onset to scar formation. Fibroblasts were isolated from the infarct region of C57BL/6J male mice (3-6 months old, n = 60) at days 0 (no MI control) and 1, 3, or 7 after MI. Whole transcriptome analysis was performed by RNA-sequencing. Of the genes sequenced, 3371 were differ  ...[more]

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