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MS for identifying CENP-E ubiquitination sites and MS for GST-Ub4 interacting proteins

ABSTRACT: LUBAC mediates the linear ubiquitination of CENP-E and facilitates the anchoring of CENP-E by KNL1 at attached kinetochores

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yan Chang

LAB HEAD: Huiyan Li

PROVIDER: PXD011786 | Pride | 2019-01-28

REPOSITORIES: Pride

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LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores.

Wu Min M Chang Yan Y Hu Huaibin H Mu Rui R Zhang Yucheng Y Qin Xuanhe X Duan Xiaotao X Li Weihua W Tu Haiqing H Zhang Weina W Wang Guang G Han Qiuying Q Li Ailing A Zhou Tao T Iwai Kazuhiro K Zhang Xuemin X Li Huiyan H

Nature communications 20190117 1

Faithful chromosome segregation requires proper chromosome congression at prometaphase and dynamic maintenance of the aligned chromosomes at metaphase. Chromosome missegregation can result in aneuploidy, birth defects and cancer. The kinetochore-bound KMN network and the kinesin motor CENP-E are critical for kinetochore-microtubule attachment and chromosome stability. The linear ubiquitin chain assembly complex (LUBAC) attaches linear ubiquitin chains to substrates, with well-established roles i ...[more]

PMID: 30655516

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