Proteomics

Dataset Information

Global Enrichment of Low-Abundance Peptide Hormones in Plasma for Discovery and Quantitation

ABSTRACT: We describe a method for global enrichment of active peptide hormones from blood plasma for both new hormone discovery and quantitative analysis. To extend the dynamic range of plasma proteomics, we employed high-resolution size exclusion chromatography to purify the peptide hormones and a powerful precipitation method to dissociate and remove bulk proteins prior to separation. Combining this method with high-sensitivity proteomic analysis, we characterized hormone abundance changes after intermittent fasting in humans.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Mark Larance  

LAB HEAD: Mark Colin Larance

PROVIDER: PXD011797 | Pride | 2019-07-16

REPOSITORIES: Pride

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20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP1.raw Raw
20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP2.raw Raw
20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP3.raw Raw
20180702_QEHF_ML_DH_EXP124_10pmoles_PRM_REP1.raw Raw
20180702_QEHF_ML_DH_EXP124_10pmoles_PRM_REP2.raw Raw
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