Proteomics

Dataset Information

0

Quantitative Analysis of Global Proteome in Arabidopsis thaliana


ABSTRACT: In total, 5,840 protein groups were identified, among which 5,188 proteins were quantified. The fold-change cutoff was set when proteins with quantitative ratios above 1.5 or below 0.667 are deemed significant. Among the quantified proteins, 95 proteins are up-regulated and 155 proteins are down-regulated in group gr1-seedling/WT-seedling.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: xiaomei li  

LAB HEAD: lirong sun

PROVIDER: PXD011814 | Pride | 2019-11-12

REPOSITORIES: Pride

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Publications

Transcriptome-wide analysis of pseudouridylation of mRNA and non-coding RNAs in Arabidopsis.

Sun Lirong L   Xu Yuxing Y   Bai Shenglong S   Bai Xue X   Zhu Huijie H   Dong Huan H   Wang Wei W   Zhu Xiaohong X   Hao Fushun F   Song Chun-Peng CP  

Journal of experimental botany 20191001 19


Pseudouridine (Ψ) is widely distributed in mRNA and various non-coding RNAs in yeast and mammals, and the specificity of its distribution has been determined. However, knowledge about Ψs in the RNAs of plants, particularly in mRNA, is lacking. In this study, we performed genome-wide pseudouridine-sequencing in Arabidopsis and for the first time identified hundreds of Ψ sites in mRNA and multiple Ψ sites in non-coding RNAs. Many predicted and novel Ψ sites in rRNA and tRNA were detected. mRNA was  ...[more]

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