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LDL Profile; a new protocol for isolation and characterization of low density lipoproteins.

ABSTRACT: This poject describe a new fast method for the LDL isolation.

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Juan Pablo Reyes Grajeda

LAB HEAD: Juan Pablpo Reyes-Grajeda

PROVIDER: PXD011843 | Pride | 2022-03-08

REPOSITORIES: Pride

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Dystrophin isoform Dp71eΔ71 is involved in the neurite outgrowth and neuronal differentiation of PC12 cells

Project description:The Dp71 protein is the most abundant dystrophin in the central nervous system (CNS). Several dystrophin Dp71 isoforms have been described and are classified into three groups, each with a different C- terminal end. However, the functions of Dp71 isoforms remain unknown. In the present study, we analyse the effect of Dp71eΔ71 overexpression on the neuronal differentiation of PC12 Tet-On cells. Overexpression of the dystrophin Dp71eΔ71 stimulates neuronal differentiation, increasing the percentage of cells with neurites and the neurite length. According to the 2-DE analysis, Dp71eΔ71 overexpression modified the protein expression profile of PC12 Tet-On cells that had been treated with neuronal growth factor (NGF) for nine days. Interestingly, all differentially expressed proteins were up-regulated compared to the control. The proteomic analysis showed that Dp71eΔ71 increases the expression of proteins with important roles in the differentiation process, such as the HspB1, S100A6, and K8 proteins involved in the cytoskeletal structure and the HCNP protein involved in neurotransmitter synthesis. The expression of the neuronal marker TH was also up- regulated.

2018-04-09 | PXD009114 | Pride