Proteomics

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Human osteoblasts quantitative proteomic analysis after cocultivation with endothelial cells


ABSTRACT: We previously described that the coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves the differentiation of both cell types, leading to the formation of functional blood vessels and enhanced bone regeneration. The objective of this study was to further delineate the multifaceted interactions between both cell types. To investigate the proteome of hOBs after cocultivation with HUVECs we used stable isotope labeling by amino acids in cell culture

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast, Cell Culture

SUBMITTER: Oscar Winninger  

LAB HEAD: Günter Finkenzeller

PROVIDER: PXD011844 | Pride | 2018-12-03

REPOSITORIES: Pride

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Publications

Increased differentiation and production of extracellular matrix components of primary human osteoblasts after cocultivation with endothelial cells: A quantitative proteomics approach.

Simunovic F F   Winninger O O   Strassburg S S   Koch H G HG   Finkenzeller G G   Stark G B GB   Lampert F M FM  

Journal of cellular biochemistry 20180820 1


Coculturing of bone-forming and blood vessel-forming cells is a strategy aimed at increasing vascularity of implanted bone constructs in tissue-engineering applications. We previously described that the coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves the differentiation of both cell types, leading to the formation of functional blood vessels and enhanced bone regeneration. The objective of this study was to further delineate the multifac  ...[more]

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