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Human Arthritic Synovial Fluid LC-MS/MS

ABSTRACT: In the present study, synovial fluid (SF) samples from inflammatory arthritis (IA) patients were subjected to a label-free, mass spectrometry-based approach for the identification and quantification of IA-specific proteins and peptides. The discovery-phase project generated a warehouse of proteomic and peptidomic data providing a basis for the identification of candidates with the greatest potential to serve as novel biomarkers specific to inflammatory arthritides. A total of 11 protein candidates and 15 peptide candidates, originating from 4 proteins, were selected for follow-up studies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Rheumatoid Arthritis Disease Specific Synovial Fluid

DISEASE(S): Rheumatoid Arthritis,Psoriatic Arthritis

SUBMITTER: Shalini Mahendran

LAB HEAD: Vinod Chandran

PROVIDER: PXD011872 | Pride | 2019-11-12

REPOSITORIES: Pride

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Elucidating the endogenous synovial fluid proteome and peptidome of inflammatory arthritis using label-free mass spectrometry.

Mahendran Shalini M SM Keystone Edward C EC Krawetz Roman J RJ Liang Kun K Diamandis Eleftherios P EP Chandran Vinod V

Clinical proteomics 20190530

<h4>Background</h4>Inflammatory arthritis (IA) is an immunological disorder in which loss of immune tolerance to endogenous self-antigens perpetuates synovitis and eventual destruction of the underlying cartilage and bone. Pathological changes in the joint are expected to be represented by synovial fluid (SF) proteins and peptides. In the present study, a mass spectrometry-based approach was utilized for the identification of key protein and peptide mediators of IA.<h4>Methods</h4>Age-matched SF ...[more]

PMID: 31160890

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