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Interactome of WT and mutant PUB1 in Saccharomyces cerevisiae

ABSTRACT: The yeast RNA binding protein PUB1 contains a prion-like domain (PrLD) and, when overexpressed, localizes into cytoplasmic liquid droplets and is toxic. However, truncated versions lacking the PrLD are either soluble or aggregating and do not significantly alter yeast growth. Analysis of the interactomes of WT and mutant PUB1 was analyzed to clarify the link between droplets formation and toxicity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Giulia Calloni

LAB HEAD: R. Martin Vabulas

PROVIDER: PXD011887 | Pride | 2021-02-04

REPOSITORIES: Pride

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RNA-binding and prion domains: the Yin and Yang of phase separation.

Gotor Nieves Lorenzo NL Armaos Alexandros A Calloni Giulia G Torrent Burgas Marc M Vabulas R Martin RM De Groot Natalia Sanchez NS Tartaglia Gian Gaetano GG

Nucleic acids research 20200901 17

Proteins and RNAs assemble in membrane-less organelles that organize intracellular spaces and regulate biochemical reactions. The ability of proteins and RNAs to form condensates is encoded in their sequences, yet it is unknown which domains drive the phase separation (PS) process and what are their specific roles. Here, we systematically investigated the human and yeast proteomes to find regions promoting condensation. Using advanced computational methods to predict the PS propensity of protein ...[more]

PMID: 32857852

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