Dataset Information

Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells

ABSTRACT: Analysis of 20S proteasome in various tissue, adipose derived stem cells(ADSC) or HeLa cells treated with interferon-gamma. This results are compared with LC-SRM results obtained on the same samples. Global proteomics on ADSC amplified in 5% or 20% O2.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Spleen, Adipose-derived Stem Cell, Heart, Testis, Brain, Liver, Lung, Thymus, Hela Cell, Ovary, Muscle, Colon, Bone Marrow

SUBMITTER: Thomas Menneteau

LAB HEAD: Odile Schiltz

PROVIDER: PXD011894 | Pride | 2019-02-14

REPOSITORIES: Pride

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Publications

Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled <i>Ex-vivo</i> Expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells.

Menneteau Thomas T Fabre Bertrand B Garrigues Luc L Stella Alexandre A Zivkovic Dusan D Roux-Dalvai Florence F Mouton-Barbosa Emmanuelle E Beau Mathilde M Renoud Marie-Laure ML Amalric François F Sensébé Luc L Gonzalez-de-Peredo Anne A Ader Isabelle I Burlet-Schiltz Odile O Bousquet Marie-Pierre MP

Molecular & cellular proteomics : MCP 20190130 4

The proteasome controls a multitude of cellular processes through protein degradation and has been identified as a therapeutic target in oncology. However, our understanding of its function and the development of specific modulators are hampered by the lack of a straightforward method to determine the overall proteasome status in biological samples. Here, we present a method to determine the absolute quantity and stoichiometry of ubiquitous and tissue-specific human 20S proteasome subtypes based ...[more]

PMID: 30700495

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Dataset Information

Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells

Publications

Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled <i>Ex-vivo</i> Expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells.

Similar Datasets

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