Proteomics

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Proteomics characterization of Chromosomal Common Fragile Site (CFS) - associated proteins uncovers ATRX as a regulator of CFS stability

ABSTRACT: Chromosomal Common Fragile Sites (CFSs) are conserved regions of our genome prone to break in conditions of replication stress (RS. Here, we have performed Chromatin Immunoprecipitation (ChIP) with FACND2 antibodies coupled to Mass Spectrometry to isolate CFSs from HeLa cells and identify the proteins enriched at these loci.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Stephanie Munk  

LAB HEAD: Andres Joaquin Lopez-Contreras

PROVIDER: PXD011937 | Pride | 2019-06-11

REPOSITORIES: Pride

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Proteomic characterization of chromosomal common fragile site (CFS)-associated proteins uncovers ATRX as a regulator of CFS stability.

Pladevall-Morera David D   Munk Stephanie S   Ingham Andreas A   Garribba Lorenza L   Albers Eliene E   Liu Ying Y   Olsen Jesper V JV   Lopez-Contreras Andres J AJ  

Nucleic acids research 20190901 15

Common fragile sites (CFSs) are conserved genomic regions prone to break under conditions of replication stress (RS). Thus, CFSs are hotspots for rearrangements in cancer and contribute to its chromosomal instability. Here, we have performed a global analysis of proteins that recruit to CFSs upon mild RS to identify novel players in CFS stability. To this end, we performed Chromatin Immunoprecipitation (ChIP) of FANCD2, a protein that localizes specifically to CFSs in G2/M, coupled to mass spect  ...[more]

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