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LC-MS/MS after anti-PUM1 and anti-PUM2 co-immunoprecipitation.

ABSTRACT: In order to identify the protein co-factors of PUMILIO1 and PUMILIO2 in TCam-2 cell line, co-IP of these proteins performed followed mass spectrometry analysis for protein identification. Six biological replicates of co-IP with anti-PUM1, anti-PUM2 antibodies and anti-IgG were performed. Three biological replicates were performed without RNase A treatment, and another three biological replicates of co-IPs with 100 mg/ml RNase A.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Male Germ Cell, Cell Culture

SUBMITTER: Erkut Ilaslan

LAB HEAD: Jadwiga Jaruzelska

PROVIDER: PXD011948 | Pride | 2020-04-23

REPOSITORIES: Pride

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Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model.

Smialek Maciej J MJ Ilaslan Erkut E Sajek Marcin P MP Swiercz Aleksandra A Janecki Damian M DM Kusz-Zamelczyk Kamila K Wozniak Tomasz T Kotecki Maciej M Handschuh Luiza L Figlerowicz Marek M Jaruzelska Jadwiga J Jaruzelska Jadwiga J

Cells 20200416 4

Mammalian Pumilio (PUM) proteins are sequence-specific, RNA-binding proteins (RBPs) with wide-ranging roles. They are involved in germ cell development, which has functional implications in development and fertility. Although human PUM1 and PUM2 are closely related to each other and recognize the same RNA binding motif, there is some evidence for functional diversity. To address that problem, first we used RIP-Seq and RNA-Seq approaches, and identified mRNA pools regulated by PUM1 and PUM2 prote ...[more]

PMID: 32316190

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