Proteomics

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Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2 apha signaling


ABSTRACT: Proteomic analysis of murine stress granule and G3BP1 granulome in infected cells. BV2 GFP-G3BP1 cells were either treated with 0.1 mM arsenite for 1h to induce SG assembly or infected with MNV for 9h. G3BP1 granules were enriched by sequential centrifugation and purified by immunoprecipitation using antibodies to GFP (to trap GFP-G3BP1) or IgG (as a control) followed by pull down with Protein A-conjugated epoxy Dynabeads as previously described. To characterize the identity of G3BP1 partners within these, Mass Spectrometric analysis was performed.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Microglial Cell

SUBMITTER: Valentina Iadevaia  

LAB HEAD: Nicolas Locker

PROVIDER: PXD011956 | Pride | 2019-12-12

REPOSITORIES: Pride

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Publications

Norovirus infection results in eIF2α independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation.

Brocard Michèle M   Iadevaia Valentina V   Klein Philipp P   Hall Belinda B   Lewis Glenys G   Lu Jia J   Burke James J   Willcocks Margaret M MM   Parker Roy R   Goodfellow Ian G IG   Ruggieri Alessia A   Locker Nicolas N  

PLoS pathogens 20200106 1


Viral infections impose major stress on the host cell. In response, stress pathways can rapidly deploy defence mechanisms by shutting off the protein synthesis machinery and triggering the accumulation of mRNAs into stress granules to limit the use of energy and nutrients. Because this threatens viral gene expression, viruses need to evade these pathways to propagate. Human norovirus is responsible for gastroenteritis outbreaks worldwide. Here we examined how norovirus interacts with the eIF2α s  ...[more]

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