Post-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Synapt MS
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Permanent Cell Line Cell, Cell Culture
DISEASE(S): Cervix Carcinoma
SUBMITTER: 
Mateusz Lacki
LAB HEAD: Stefan Tenzer
PROVIDER: PXD011970 | Pride | 2019-05-28
REPOSITORIES: Pride
Publications
Enhancing Sensitivity of Microflow-Based Bottom-Up Proteomics through Postcolumn Solvent Addition.
Distler Ute U Łącki Mateusz Krzysztof MK Schumann Sven S Wanninger Markus M Tenzer Stefan S
Analytical chemistry 20190604 12
The introduction of more sensitive mass spectrometers allows researchers to adapt front-end liquid chromatography (LC) to individual needs for the analysis of complex proteomes. Where absolute sensitivity is not paramount, it is advantageous to switch from a highly sensitive nanoflow-LC setup, the de facto standard platform in mass-spectrometry (MS)-based discovery proteomics, to a more robust, high-throughput-compatible microflow or conventional-flow setup. To enhance the microflow-LC-MS electr ...[more]
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