Proteomics

Dataset Information

Post-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics

ABSTRACT: We demonstrate that post-column addition of organic solvents leads to a strong enhancement of electrospray stability in microflow LC-MS experiments improving the sensitivity across the entire gradient, for early eluting peptides by up to tenfold. Post-column solvent addition did not negatively affect chromatographic performance and resulted in an overall 28%-36% increase in identifications at both protein- and peptide-level. Post-column solvent addition can be easily adopted on any microflow LC-MS/MS system.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Mateusz Lacki  

LAB HEAD: Stefan Tenzer

PROVIDER: PXD011970 | Pride | 2019-05-28

REPOSITORIES: Pride

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2018-04_MF_PC_vs_noPC_userdesigned20181024-155736_peptide_quantification_report.xlsx Xlsx
2018-04_MF_PC_vs_noPC_userdesigned20181024-155736_quantification_report.xlsx Xlsx
2018-04_Microflow_final_runs_135_25_750_120min_251_peptide_quantification_report.xlsx Xlsx
2018-04_Microflow_final_runs_135_25_750_120min_251_quantification_report.xlsx Xlsx
2018-04_Microflow_final_runs_135_25_750_35min_0_5ug_251_peptide_quantification_report.xlsx Xlsx
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