Proteomics

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Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids


ABSTRACT: Methyl ketone production by P. putida with A. thaliana and switchgrass hydrolysates obtained by dilute acid pretreatment led to the identification of plant-derived amino acids, rather than mono-aromatics, as key stimulative components of these hydrolysates. Shotgun proteomics indicated that the amino acids had a specific inductive effect on proteins involved in fatty acid biosynthesis, leading to a 9-fold increase in methyl ketone titer when amending glucose-containing minimal medium with a defined set of amino acids.

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Pseudomonas putida KT2440  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Christopher Petzold  

LAB HEAD: Christopher J. Petzold

PROVIDER: PXD012013 | Pride | 2019-06-27

REPOSITORIES: Pride

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Publications


Plants are an attractive sourceof renewable carbon for conversion to biofuels and bio-based chemicals. Conversion strategies often use a fraction of the biomass, focusing on sugars from cellulose and hemicellulose. Strategies that use plant components, such as aromatics and amino acids, may improve the efficiency of biomass conversion. Pseudomonas putida is a promising host for its ability to metabolize a wide variety of organic compounds. P. putida was engineered to produce methyl ketones, whic  ...[more]

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