Proteomics

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Fasting, FDFT1 expression and colorectal cancer


ABSTRACT: First, lentivirus-mediated overexpression of FDFT1 and lentivirus-mediated knockdown of FDFT1 were performed in CT26 cells. Then control CT26 cells, FDFT1 overexpressing CT26 cells and FDFT1 knockdown CT26 cells were cultured under normal medium or fasting mimic medium. Fasting mimic medium was done by incubating cells in glucose-free DMEM (Gibco, USA) supplemented with 0.5g/L glucose and 1% FBS for 48h. So we have 6 groups: control CT26 cells, FDFT1 overexpressing CT26 cells, FDFT1 knockdown CT26 cells, control CT26 cells-under fasting mimic medium, FDFT1 overexpressing CT26 cells- under fasting mimic medium, FDFT1 knockdown CT26 cells- under fasting mimic medium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Xiao Zhen  

LAB HEAD: Meilin Weng

PROVIDER: PXD012029 | Pride | 2020-03-19

REPOSITORIES: Pride

Dataset's files

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Action DRS
Hcc-1.mgf Mgf
Hcc-1.raw Raw
Hcc-10.mgf Mgf
Hcc-10.raw Raw
Hcc-11.mgf Mgf
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Publications

Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression.

Weng Mei-Lin ML   Chen Wan-Kun WK   Chen Xiang-Yuan XY   Lu Hong H   Sun Zhi-Rong ZR   Yu Qi Q   Sun Peng-Fei PF   Xu Ya-Jun YJ   Zhu Min-Min MM   Jiang Nan N   Zhang Jin J   Zhang Jian-Ping JP   Song Yuan-Lin YL   Ma Duan D   Zhang Xiao-Ping XP   Miao Chang-Hong CH   Miao Chang-Hong CH  

Nature communications 20200420 1


Evidence suggests that fasting exerts extensive antitumor effects in various cancers, including colorectal cancer (CRC). However, the mechanism behind this response is unclear. We investigate the effect of fasting on glucose metabolism and malignancy in CRC. We find that fasting upregulates the expression of a cholesterogenic gene, Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1), during the inhibition of CRC cell aerobic glycolysis and proliferation. In addition, the downregulation of FDFT1 i  ...[more]

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