Analysis of FucT and XylT1B mutants in C. reinhardtii

ABSTRACT: Secreted proteins found in the supernatant of different mutants were analyzed by mass spectrometry employing In source-CID. After analysis of intact N-glycopeptides on MS1 level, N-glycan compositions found were compared between two strains, consequently unraveling the molecular function of enzymes knocked down. Hereby, the role of a putative fucosyltransferase and the identity of a second core- xylosyltransferase could be confirmed.

INSTRUMENT(S):

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Culture Supernatant, Photosynthetic Cell

SUBMITTER: Anne Oltmanns  

LAB HEAD: Michael Hippler

PROVIDER: PXD012107 | Pride | 2019-11-19

REPOSITORIES: Pride