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Quantitative proteomics profiling revealed serum exosomes inhibited the differentiation of osteoclast in SAPHO patients during the active period

ABSTRACT: The serum samples from patients and normal volunteers were pooled together, respectively, to perform isolation of exosomes by ultracentrifugation.TMT-labeled quantitative proteomics were performed to find out the differently expressed proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Wei Ge

LAB HEAD: Wei Ge

PROVIDER: PXD012125 | Pride | 2021-09-08

REPOSITORIES: Pride

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Serum-derived extracellular vesicles inhibit osteoclastogenesis in active-phase patients with SAPHO syndrome.

Gao Yanpan Y Chen Yanyu Y Wang Lun L Li Chen C Ge Wei W

Therapeutic advances in musculoskeletal disease 20210416

<h4>Objective</h4>Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome is a rare chronic inflammatory disorder and the underlying pathogenesis is unclear. In this study, 88 SAPHO patients and 118 healthy controls were recruited to investigate the role of serum-derived extracellular vesicles (SEVs) in SAPHO syndrome.<h4>Methods</h4>Quantitative proteomics was applied for SEVs proteome identification, and ELISA and Western blotting was performed to verify the results of mass sp ...[more]

PMID: 33948126

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