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Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells

ABSTRACT: Sensitivity of cumulus enclosed-oocytes to metabolic stress during in vitro maturation. A proteomic insight

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cumulus Cell, Oocyte

SUBMITTER: Kurt Boonen

LAB HEAD: Jo .L.M.R. Leroy

PROVIDER: PXD012141 | Pride | 2019-04-02

REPOSITORIES: Pride

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Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells.

Marei Waleed F A WFA Van Raemdonck Geert G Baggerman Geert G Bols Peter E J PEJ Leroy Jo L M R JLMR

Scientific reports 20190306 1

Maternal lipolytic metabolic disorders result in a lipotoxic microenvironment in the ovarian follicular fluid (FF) which deteriorates oocyte quality. Although cellular stress response mechanisms are well defined in somatic cells, they remain largely unexplored in oocytes, which have distinct organelle structure and nuclear transcription patterns. Here we used shotgun proteomic analyses to study cellular responses of bovine oocytes and cumulus cells (CCs) after in vitro maturation under lipotoxic ...[more]

PMID: 30842615

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