Dataset Information

Gut anatomical development and microbial functional assembly of wood-feeding beetles

ABSTRACT: Proteomic analysis of the microbiome of beetle intestinal content from wood eating beetles as related to lignocellulose deconstruction and colony subsistence

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Odontotaenius Disjunctus

TISSUE(S): Intestinal Juice

SUBMITTER: Matthew Monroe

LAB HEAD: Joshua Adkins

PROVIDER: PXD012200 | Pride | 2019-01-18

REPOSITORIES: Pride

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Publications

Gut anatomical properties and microbial functional assembly promote lignocellulose deconstruction and colony subsistence of a wood-feeding beetle.

Ceja-Navarro Javier A JA Karaoz Ulas U Bill Markus M Hao Zhao Z White Richard A RA Arellano Abelardo A Ramanculova Leila L Filley Timothy R TR Berry Timothy D TD Conrad Mark E ME Blackwell Meredith M Nicora Carrie D CD Kim Young-Mo YM Reardon Patrick N PN Lipton Mary S MS Adkins Joshua N JN Pett-Ridge Jennifer J Brodie Eoin L EL

Nature microbiology 20190311 5

Beneficial microbial associations enhance the fitness of most living organisms, and wood-feeding insects offer some of the most striking examples of this. Odontotaenius disjunctus is a wood-feeding beetle that possesses a digestive tract with four main compartments, each of which contains well-differentiated microbial populations, suggesting that anatomical properties and separation of these compartments may enhance energy extraction from woody biomass. Here, using integrated chemical analyses, ...[more]

PMID: 30858574

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Project description:Wood-degrading brown rot fungi are essential recyclers of plant biomass in forest ecosystems. Their efficient cellulolytic systems, which have potential biotechnological applications, apparently depend on a combination of two mechanisms: lignocellulose oxidation by reactive oxygen species (ROS) and polysaccharide hydrolysis by a limited set of glycoside hydrolases (GHs). Given that ROS are strongly oxidizing and non-selective, these two steps are likely segregated. A common hypothesis has been that brown rot fungi use a concentration gradient of chelated metal ions to confine ROS generation inside wood cell walls before enzymes can infiltrate. We examined an alternative: that lignocellulose-oxidation (LOX) components involved in ROS production are differentially expressed by brown rot fungi ahead of GH components. We used spatial mapping to resolve a temporal sequence in Postia placenta, sectioning thin wood wafers colonized directionally. Among sections, we measured gene expression by whole transcriptome sequencing (RNAseq) and assayed relevant enzyme activities. We found a marked pattern of LOX upregulation in a narrow (5-mm; 48-hr) zone at the hyphal front, which included many genes likely involved in ROS generation. Upregulation of GH5 endoglucanases and many other GHs clearly occurred later, behind the hyphal front, with notable exceptions of two likely expansins and a GH28 pectinase. Our results support a staggered mechanism for brown rot that is controlled by differential expression rather than microenvironmental gradients. This mechanism likely results in an oxidative pretreatment of lignocellulose, possibly facilitated by expansin- and pectinase-assisted cell wall swelling, before cellulases and hemicellulases are deployed for polysaccharide depolymerization. We sequenced mRNA from 9 Postia placenta samples taken from 3 wood sections of wafer design, with 3 bioreplicates for each wood section, to compare the gene expression during brown rot processes. Three wood sections of the wafer are representing early to late decay stages.

Dataset Information

Gut anatomical development and microbial functional assembly of wood-feeding beetles

Publications

Gut anatomical properties and microbial functional assembly promote lignocellulose deconstruction and colony subsistence of a wood-feeding beetle.

Similar Datasets

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