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Analysis of mDiaphanous1 (mDia1) interactome in adult mouse skeletal muscle C2C12myoblasts and myotubes

ABSTRACT: LC-MS/MS analysis of mDia1 interacting proteins in myoblasts and myotubes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Skeletal Muscle Myoblast In Skeletal Muscle

SUBMITTER: Gunasekaran Subramaniam

LAB HEAD: Prof. Jyotsna Dhawan

PROVIDER: PXD012257 | Pride | 2019-06-06

REPOSITORIES: Pride

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Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation.

Saleh Amena A Subramaniam Gunasekaran G Raychaudhuri Swasti S Dhawan Jyotsna J

Scientific reports 20190605 1

Muscle differentiation is controlled by adhesion and growth factor-dependent signalling through common effectors that regulate muscle-specific transcriptional programs. Here we report that mDiaphanous1, an effector of adhesion-dependent RhoA-signalling, negatively regulates myogenesis at the level of Myogenin expression. In myotubes, over-expression of mDia1ΔN3, a RhoA-independent mutant, suppresses Myogenin promoter activity and expression. We investigated mDia1-interacting proteins that may co ...[more]

PMID: 31165762

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