Dataset Information

Quantitative protein profiling of brain vessels in rhesus macaque

ABSTRACT: Aging is a major risk factor for cerebrovascular disease. However, the molecular mechanisms of cerebrovascular aging remain to be clarified. In this study, we performed proteomics analysis aimed to reveal the molecular signaling pathways during aging.our study demonstrated that the imbalance of vasodilators and vasoconstrictors caused by downregulation of NOS3, oxidative stress mediated by the suppression of NRF2 pathway, and miRNA-regulated accumulation of ECM proteins were critical for cerebrovascular aging.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Macaca Mulatta (rhesus Macaque)

TISSUE(S): Blood Vessel

SUBMITTER: Wei Ge

LAB HEAD: Wei Ge

PROVIDER: PXD012306 | Pride | 2019-11-13

REPOSITORIES: Pride

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Publications

Proteome Profiling of Cerebral Vessels in Rhesus Macaques: Dysregulation of Antioxidant Activity and Extracellular Matrix Proteins Contributes to Cerebrovascular Aging in Rhesus Macaques.

Wang Xia X Liu Yifan Y Jia Yangjie Y Liu Haotian H Bao Xinjie X He Zhanlong Z Ge Wei W

Frontiers in aging neuroscience 20191023

Aging is a major risk factor for cerebrovascular disease; however, the molecular mechanisms of cerebrovascular aging remain to be clarified. The aim of this study was to reveal the molecular signaling pathways involved in cerebrovascular aging. This study used high-resolution liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), in combination with quantitative 6-plex tandem mass tag labeling, to profile protein changes in brain vessels from three groups of healthy rhesus macaque ...[more]

PMID: 31708766

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Project description:The molecular basis of complex neuropsychiatric disorders most likely involves many genes. In recent years, specific genetic variations influencing risk for schizophrenia and other neuropsychiatric disorders have been reported. We have used custom DNA microarrays and qPCR to investigate the expression of putative schizophrenia susceptibility genes and related genes of interest in the normal human brain. Expression of 31 genes was measured in Brodmann's area 10 (BA10) in the prefrontal cortex of 72 postmortem brain samples spanning half a century of human aging (18-67 years), each without history of neuropsychiatric illness, neurological disease, or drug abuse. Examination of expression across age allowed the identification of genes whose expression patterns correlate with age, as well as genes that share common expression patterns and that possibly participate in common cellular mechanisms related to the emergence of schizophrenia in early adult life. The expression of GRM3 and RGS4 decreased across the entire age range surveyed, while that of PRODH and DARPP-32 was shown to increase with age. NRG1, ERBB3, and NGFR show expression changes during the years of greatest risk for the development of schizophrenia. Expression of FEZ1, GAD1, and RGS4 showed especially high correlation with one another, in addition to the strongest mean levels of absolute correlation with all other genes studied here. Keywords: postmortem human brain collection Supplementary files: Gene expression measures in Matrix1379Normalized.txt include all data from the 1379 probes on the arrays, and are NOT adjusted for PMI, brain pH, and RIN, and are NOT averaged over multiple probes within genes. Raw data are located in Raw_1241842_gene_probe_profile.txt. We have used custom DNA microarrays to investigate gene expression in the normal human brain. Expression was measured in Brodmann's area 10 (BA10) in the prefrontal cortex of 72 postmortem brain samples spanning half a century of human aging (18-67 years), each without history of neuropsychiatric illness, neurological disease, or drug abuse.

Dataset Information

Quantitative protein profiling of brain vessels in rhesus macaque

Publications

Proteome Profiling of Cerebral Vessels in Rhesus Macaques: Dysregulation of Antioxidant Activity and Extracellular Matrix Proteins Contributes to Cerebrovascular Aging in Rhesus Macaques.

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