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Analalysis of ASPP2 interaction

ABSTRACT: Quantitative AP-MS from HEK 293T cells expressing Strep-SHA tagged ASPP2 or ASPP2. (Ex1) Quantitative AP-MS from HEK293 cells expressing Strep-SHA tagged PP1alpha, beta, gamma. (Ex2)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell

SUBMITTER: Federico Uliana

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD012378 | Pride | 2019-04-02

REPOSITORIES: Pride

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ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail.

Bertran M Teresa MT Mouilleron Stéphane S Zhou Yanxiang Y Bajaj Rakhi R Uliana Federico F Kumar Ganesan Senthil GS van Drogen Audrey A Lee Rebecca R Banerjee Jennifer J JJ Hauri Simon S O'Reilly Nicola N Gstaiger Matthias M Page Rebecca R Peti Wolfgang W Tapon Nicolas N

Nature communications 20190215 1

Serine/threonine phosphatases such as PP1 lack substrate specificity and associate with a large array of targeting subunits to achieve the requisite selectivity. The tumour suppressor ASPP (apoptosis-stimulating protein of p53) proteins associate with PP1 catalytic subunits and are implicated in multiple functions from transcriptional regulation to cell junction remodelling. Here we show that Drosophila ASPP is part of a multiprotein PP1 complex and that PP1 association is necessary for several ...[more]

PMID: 30770806

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