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Differentially expressed N-glycopeptides in HepG2 related to LO2 cell line

ABSTRACT: With isotopically dimethyl labeling, ZIC-HILIC enrichment, C18 pre-fractionation, differentially expressed N-glycosylation in HCC HepG2 cells (relative to normal liver LO2 cells) were analyzed using penta-HILIC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Kaijie Xiao

LAB HEAD: Zhixin Tian

PROVIDER: PXD012424 | Pride | 2019-05-31

REPOSITORIES: Pride

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GPSeeker Enables Quantitative Structural N-Glycoproteomics for Site- and Structure-Specific Characterization of Differentially Expressed N-Glycosylation in Hepatocellular Carcinoma.

Xiao Kaijie K Tian Zhixin Z

Journal of proteome research 20190603 7

N-Glycosylation, being one of the most common and complex protein post-translational modifications (PTMs), is known to have microheterogeneity with the presence of different N-glycan structures at a single specific glycosite. These different structures may have exactly the same monosaccharide composition but totally different differential expressions and pathological relevance. Mass spectrometry-based N-glycoproteomics has so far been successful in large-scale characterization of these N-glycans ...[more]

PMID: 31117584

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